论文库数据----深海科学与工程研究所

当前位置：首页 > 学术成果 > 2023 > 论文

2023

论文库

论文题目	Wnt Signaling Pathway Collapse upon beta-Catenin Destruction by a Novel Antimicrobial Peptide SKACP003: Unveiling the Molecular Mechanism and Genetic Activities Using Breast Cancer Cell Lines
论文题目（英文）	Wnt Signaling Pathway Collapse upon beta-Catenin Destruction by a Novel Antimicrobial Peptide SKACP003: Unveiling the Molecular Mechanism and Genetic Activities Using Breast Cancer Cell Lines
作者	Selvarathinam, Kanitha(1);Subramani, Prabhu(2);Thekkumalai, Malarvili(3);Vilwanathan, Ravikumar(2);Selvarajan, Ramganesh(4,5);Abia, Akebe Luther King(4,6)
发表年度	2023-02
卷	28
期	3
页码
期刊名称	MOLECULES
摘要
摘要_英文	Despite progress in breast cancer treatment, the survival rate for patients with metastatic breast cancer remains low due to chemotherapeutic agent resistance and the lack of specificity of the current generation of cancer drugs. Our previous findings indicated that the antimicrobial peptide SKACP003 exhibited anticancer properties, particularly against the MCF-7, MDA-MB-231, and MDA-MB-453 breast cancer cell lines. However, the mechanism of SKACP003-induced cancer cell death is unknown. Here, we investigated the molecular mechanism by which SKACP003 inhibits the cell cycle, cell proliferation, and angiogenesis in breast cancer cell lines. The results revealed that all the breast cancer cell lines treated at their IC50 values significantly inhibited the replicative phase of the cell cycle. The SKACP003-induced growth inhibition induced apoptosis, as evidenced by a decrease in BCL-2 and an increase in BAX and caspase gene (Cas-3, Cas-8, and Cas-9) expression. Reduced expression of the beta-Catenin signaling pathway was associated with the SKACP003-induced apoptosis. SKACP003-treated breast cancer cells showed decreased expression of Wnt/beta-Catenin targeting genes such as C-Myc, P-68, and COX-2 and significant downregulation of CDK-4 and CDK-6 genes. Furthermore, cytoplasmic beta-catenin protein levels in SKACP003-treated cell lines were significantly lower than in control cell lines. The results of the current study suggest that the newly identified antimicrobial peptide SKACP003 has great potential as a candidate for specifically targeting the beta-catenin and thus significantly reducing the progression and prognosis of breast cancer cell lines.